About eFluor 514 Calcium Sensor

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eFluor™ 514 (eF514) Calcium Sensor is a green-emitting calcium sensing dye that can be excited by the 488 nm blue laser and captured with a 525/50 bandpass filter. eF514 has an excitation peak at 490 nm and an emission peak at 514 nm, and is very similar spectrally and functionally to Fluo-3 AM and Fluo-4 AM, which are also calcium sensing dyes. This is a membrane-permeable calcium sensing dye that is used to monitor changes in calcium concentrations within the cell. eF514 Calcium Sensor can be used with fluorescence microscopy and microplate assay applications. This dye is part of the larger eFluor™ dye family, developed by eBioscience which is now part of ThermoFisher Scientific.

Alternative Dyes to eFluor 514 Calcium Sensor

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Fluo-4

Dyes similar to eFluor 514 Calcium Sensor based on emission

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5-CFDA 5-FAM ATTO 465 ATTO 488 AccuClear Acridine Orange Aldefluor Alexa Fluor 488 Brilliant Blue 515 BDY FL SE BDY FL-X SE BODIPY 500/510 BODIPY FL BactoView Live Green Brilliant Violet 510 CFSE CF440 CF488A CF500 CPN510 Green Calcein Calcein AM Calcein Green Carboxyfluorescein CellBrite Fix 488 CellBrite Green CellROX Green CellTracker Violet Chromeo 488 FITC Plus CoraLite Plus 488 Cy2 CytoTrace Green CMFDA CytoTrack Green DY-380XL DyLight 488 EFLUXX-ID Green Emerald GFP ExoBrite 490/515 EV Membrane Stain FDG FLUOFORTE FVS510 FVS520 Flash Phalloidin Green 488 Fluo-4 Fluo-4 AM Fluorescein Fura-2 (Ca2+bound) Fura-2 (Ca2+free) GFP Ghost Dye Blue 516 Ghost Dye Violet 510 KIRAVIA Blue 520 Kaede (green) KikGR1 (green) Live-or-Dye 488/515 Live/Dead Fix Green Live/Dead Fix Scarlet LysoBrite Green LysoTracker Green Mag-Fluo-4 MagDot 525 MaxLight 490 MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green FM MitoTracker Green MitoView Green MultiDot 525 NL 493 Neuro DiO NeuroVue Jade NovaFluor Blue 510 NucSpot Live 488 Oregon Green 488 BAPTA Oxazole Yellow Oxazole Yellow Homodimer Oyster 488 PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fura-2 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Fluor 488 PromoFluor 488P ReadiLink 405-508 ReadiLink 492-516 Rhodamine 110 Rhodamine Green STAR 440L STAR 440SXP STAR GREEN SYBR Green Seta-385-NHS Spark Blue 515 Spectrum Green StarBright UltraViolet 510 StarBright Violet 515 SureLight 488 Sytox Green T-Sapphire TFAX 488 SE TFAX 488 TFP TSA Vivid 520 Thiazole Green VRDye 490 Viafluor CFSE Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye Vio515 VioBright 515 VioBright FITC VioGreen VivaFix 408/512 VivaFix 498/521 YO-PRO-1 YOYO-1 Zombie Aqua Zombie Green ZsGreen eFluor 506 eFluor 520 Fix Viability eGFP iFluor 488 mAmetrine BDY FL Staurosporine BDY Lenalidomide