About Live-or-Dye 488/515

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Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 488/515 has an excitation peak at 488 nm and an emission peak at 515 nm.

Alternative Dyes to Live-or-Dye 488/515

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FVS520 Ghost Dye Blue 516 Zombie Green VivaFix 498/521

Dyes similar to Live-or-Dye 488/515 based on emission

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5-CFDA 5-FAM ATTO 465 ATTO 488 AccuClear Acridine Orange Aldefluor Alexa Fluor 488 Brilliant Blue 515 BCECF (525em) pH9.0 BCECF BCECF (Red em) pH5.2 BCECF AM BDY FL SE BDY FL-X SE BODIPY 500/510 BODIPY FL BactoView Live Green Brilliant Violet 510 CFSE CF440 CF488A CF500 CPN510 Green Calcein Calcein AM Calcein Green Carboxyfluorescein CellBrite Fix 488 CellBrite Steady 488 CellROX Green CellTrace Oregon Green CellTracker Violet Chromeo 488 FITC Plus CoraLite Plus 488 Cy2 CytoTrace Green CMFDA CytoTrack Green DCF DY-380XL DyLight 488 EFLUXX-ID Green Emerald GFP ExoBrite 490/515 EV Membrane Stain FDG FITC FLUOFORTE FVS520 Flash Phalloidin Green 488 Fluo-4 Fluo-4 AM Fluorescein Fura-2 (Ca2+bound) Fura-2 (Ca2+free) GFP Ghost Dye Blue 516 Ghost Dye Violet 510 HiLyte 488 KIRAVIA Blue 520 Kaede (green) KikGR1 (green) Live/Dead Fix Aqua Live/Dead Fix Green Live/Dead Fix Scarlet LysoBrite Green LysoTracker Green Mag-Fluo-4 MagDot 525 MaxLight 490 MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green EX488 MitoLite Green FM MitoTracker Green MitoView Green MultiDot 525 NL 493 NeuroVue Jade NovaFluor Blue 510 NucSpot Live 488 Oregon Green 488 Oregon Green 488 BAPTA Oxazole Yellow Homodimer Oyster 488 PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Fluor 488 Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P Qdot 525 ROS-ID Green ReadiLink 405-508 ReadiLink 492-516 Rhodamine 110 Rhodamine Green STAR 440L STAR 440SXP STAR 488 STAR GREEN SYBR Green Seta-385-NHS Spark Blue 515 Spectrum Green StarBright UltraViolet 510 StarBright Violet 515 SureLight 488 Syto 16 Sytox Green T-Sapphire TFAX 488 SE TFAX 488 TFP TSA Vivid 520 Thiazole Green VRDye 490 Viafluor CFSE Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye Vio515 VioBright 515 VioBright FITC VioGreen VivaFix 408/512 VivaFix 498/521 YO-PRO-1 YOYO-1 Zombie Aqua Zombie Green ZsGreen eFluor 514 Calcium Sensor eFluor 520 Fix Viability eGFP iFluor 488 mAmetrine BDY FL Staurosporine BDY Lenalidomide