About CFSE

---

Carboxyfluorescein diacetate succinimidyl ester (CFSE) is a blue-emitting amine-reactive dye that can be excited by the 405 nm Violet laser and captured using a 530/43 bandpass filter. It has excitation peak at 494 nm and and an emission peak at 521 nm. CFSE is most commonly used for in vivo cell tracking and proliferation studies. When a cell reproduces, the dye is distributed evenly to daughter cells , with fluorescence intensity halving with each successive passage. This is a generic dye and it can be found in kits from various suppliers.

Alternative Dyes to CFSE

Check to add to spectra viewer
CytoTrack Green CFSE

Dyes similar to CFSE based on emission

Check to add to spectra viewer

5-CFDA 5-FAM ATTO 488 Acridine Orange Aldefluor Alexa Fluor 488 Brilliant Blue 515 BCECF (525em) pH9.0 BCECF BCECF (Red em) pH5.2 BCECF AM BODIPY 500/510 BactoView Live Green CF440 CF488A Calcein Green Calcium Green Carboxyfluorescein CellBrite Fix 488 CellBrite Steady 488 CellROX Green CellTrace Oregon Green CellTracker Violet Chromeo 488 Chromeo 505 Citrine FITC Plus CoraLite Plus 488 CytoTrace Green CMFDA CytoTrack Green DCF DMAO DyLight 488 EFLUXX-ID Green EvaGreen Plus ExoBrite 490/515 EV Membrane Stain FDG FITC FLUOFORTE FVS520 Flash Phalloidin Green 488 Fluo-3 Fluo-3 AM Fluo-4 Fluo-4 AM Fluorescein GOLGI-ID Green GelGreen Ghost Dye Blue 516 HiLyte 488 KIRAVIA Blue 520 Kaede (green) KikGR1 (green) Krome Orange LIVE 510 Live-or-Dye 488/515 Live/Dead Fix Aqua Live/Dead Fix Green LysoView 488 Mag-Fluo-4 MagDot 525 MaxLight 490 MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green EX488 MitoView Green MultiDot 525 NL 493 Newport Green DCF NovaFluor Blue 530 NucSpot Live 488 NucView 488 Nuclear Green Nuclear-ID Green Oregon Green 488 Oregon Green 488 BAPTA Oregon Green 514 Oyster 488 PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluo-4 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator PhenoVue Fluor 488 Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P Qdot 525 ROS-ID Green ReadiLink 492-516 Rhodamine 110 Rhodamine Green Riboflavin STAR 440SXP STAR 488 STAR 512 STAR GREEN SYBR Green Seta-385-NHS Spectrum Green StarBright Violet 515 SureLight 488 Syto 16 Sytox Green TFAX 488 SE TFAX 488 TFP TSA Vivid 520 Thiazole Green Topaz VRDye 490 Venus Viafluor CFSE Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye Vio515 VioBright 515 VioBright FITC VioGreen VivaFix 408/512 VivaFix 498/521 YFP Zombie Aqua Zombie Green ZsGreen eFluor 514 Calcium Sensor eFluor 520 Fix Viability eYFP iFluor 488 mAmetrine mCitrine BDY FL Staurosporine