CellBrite Fix 488 Dye Profile

Biotium™

488

Laser Line

515/30

Common Filter

480

Excitation Max

513

Emission Max

About CellBrite Fix 488


Biotium's CellBrite Fix dyes are versatile amine-reactive dyes that are primarily used for live cell outer plasma membrane staining but can withstand fixation and detergent permeabilization. The unique benefit here is that since CellBrite Fix dyes can withstand permeabilization, extracellular staining can be combined with intracellular staining techniques. This dye line shares similarities with Biotium's MemBrite Fix and MemBrite Fix-ST line, but there are a few key differences. CellBrite Fix stains are easy to use and require a single staining step while MemBrite Fix dyes require a two-step staining protocol. While ease of use is important, MemBrite Fix and Membrite Fix-ST dyes have more colors to choose from and they do not associate with lipids in the membrane which leads to lower cytoplasmic background after permeabilization.



Spectra



Dyes similar to CellBrite Fix 488 based on emission

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5-CFDA 5-FAM ATTO 465 AccuClear Acridine Orange Aldefluor Alexa Fluor 488 Brilliant Blue 515 BDY FL SE BDY FL-X SE BODIPY 500/510 BODIPY FL BactoView Live Green Brilliant Violet 510 CFSE CF440 CF488A CF500 CPN510 Green Calcein Calcein AM Calcein Green Carboxyfluorescein CellBrite Green CellROX Green CellTracker Violet Chromeo 488 FITC Plus CoraLite Plus 488 Cy2 CytoTrace Green CMFDA CytoTrack Green DY-380XL DiO DyLight 488 EFLUXX-ID Green Emerald GFP ExoBrite 490/515 EV Membrane Stain FDG FLUOFORTE FVS510 FVS520 Flash Phalloidin Green 488 Fluo-4 Fluo-4 AM Fluorescein Fura-2 (Ca2+bound) Fura-2 (Ca2+free) GFP Ghost Dye Blue 516 Ghost Dye Violet 510 KIRAVIA Blue 520 Kaede (green) KikGR1 (green) Live-or-Dye 488/515 Live/Dead Fix Lime Live/Dead Fix Scarlet LysoBrite Green LysoTracker Green Mag-Fluo-4 Mag-Fura-2 MagDot 525 MaxLight 490 MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green FM MitoTracker Green MultiDot 525 NL 493 Neuro DiO NeuroVue Jade NovaFluor Blue 510 NucSpot Live 488 Nuclear Yellow Oregon Green 488 BAPTA Oxazole Yellow Oxazole Yellow Homodimer Oyster 488 PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fura-2 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Fluor 488 PromoFluor 488P ReadiLink 405-508 ReadiLink 492-516 Rhodamine 110 STAR 440L STAR 440SXP STAR GREEN SYBR Green Seta-385-NHS Spark Blue 515 Spectrum Green StarBright UltraViolet 510 StarBright Violet 515 SureLight 488 T-Sapphire TFAX 488 SE TFAX 488 TFP TSA Vivid 520 Thiazole Green VRDye 490 Viafluor CFSE Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye Vio515 VioBright 515 VioBright FITC VioGreen VivaFix 408/512 VivaFix 498/521 YO-PRO-1 YOYO-1 Zombie Aqua Zombie Green ZsGreen eFluor 506 eFluor 514 Calcium Sensor eFluor 520 Fix Viability eGFP iFluor 488 mAmetrine mAzamiGreen BDY FL Staurosporine BDY Lenalidomide