About Spark Blue 515

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Spark Blue™ 515 exhibits peak excitation/emission wavelengths at 506 nm and 516 nm respectively, most closely matching the spectra of cFluor® B515. On conventional cytometers, it can be detected with a 488 nm blue laser and the 530/30 filter set typically used by FITC or Alexa Fluor® 488. On spectral instruments with the proper settings, it is possible to unmix Spark Blue™ 515 from dyes like FITC, Alexa Fluor® 488, and KIRAVIA Blue 520™ as its peak emission is shifted over 10 nm. With a dim brightness, Spark Blue™ 515 is best suited for detecting antigens with medium to high expression levels. It is also stable towards exposure to heat or a variety of commonly used fixative solutions.

Dyes similar to Spark Blue 515 based on emission

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5-CFDA 5-FAM ATTO 465 AccuClear Aldefluor Alexa Fluor 488 Brilliant Blue 515 BDY FL SE BDY FL-X SE BODIPY 493/503 BODIPY 500/510 BODIPY FL Brilliant Violet 510 CF440 CF488A CF500 CPN510 Green Calcein Calcein AM Calcein Green Carboxyfluorescein CellBrite Fix 488 CellBrite Green CellTracker Violet Chromeo 488 CoraLite Plus 488 Cy2 CytoTrace Green CMFDA DY-380XL DiO DyLight 488 EFLUXX-ID Green Emerald GFP ExoBrite 490/515 EV Membrane Stain FDG FLUOFORTE FVS510 FVS520 Fluo-4 Fluo-4 AM Fluorescein Fura-2 (Ca2+bound) Fura-2 (Ca2+free) GFP Ghost Dye Blue 516 Ghost Dye Violet 510 Kaede (green) KikGR1 (green) Live-or-Dye 488/515 Live/Dead Fix Lime Live/Dead Fix Scarlet LysoBrite Green LysoTracker Green Mag-Fluo-4 Mag-Fura-2 MagDot 525 MaxLight 490 MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green FM MitoTracker Green MultiDot 525 NL 493 Neuro DiO NeuroVue Jade NovaFluor Blue 510 NucSpot Live 488 Nuclear Yellow Oxazole Yellow Oxazole Yellow Homodimer Oyster 488 PKH67 PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fura-2 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Fluor 488 PromoFluor 488P ReadiLink 405-508 ReadiLink 492-516 STAR 440L STAR 440SXP STAR GREEN SYBR Green Seta-385-NHS Spectrum Green StarBright UltraViolet 510 StarBright Violet 515 SureLight 488 T-Sapphire TFAX 488 SE TFAX 488 TFP TSA Vivid 520 VRDye 490 Viafluor CFSE Vio515 VioBright 515 VivaFix 408/512 VivaFix 498/521 YO-PRO-1 YOYO-1 Zombie Aqua Zombie Green ZsGreen eFluor 506 eFluor 506 Fix Viability eFluor 514 Calcium Sensor eGFP iFluor 488 mAzamiGreen BDY FL Staurosporine BDY Lenalidomide