About Live/Dead Fix Lime

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The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Lime has an excitation peak at 408 nm and an emission peak at 504 nm.

Dyes similar to Live/Dead Fix Lime based on emission

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7C ATTO 465 AccuClear Aldefluor AmCyan - Protein BDY FL SE BDY FL-X SE BODIPY 493/503 BODIPY FL Brilliant Ultraviolet 496 Brilliant Violet 510 CF430 CF500 CPN510 Green Calcein Calcein AM Calcein Green CellBrite Fix 488 CellBrite Green Cy2 DY-380XL DiO EFLUXX-ID Green Emerald GFP FVS510 Fluorescein Fura-2 (Ca2+bound) Fura-2 (Ca2+free) GFP Ghost Dye Violet 510 Hoechst (33342) Live/Dead Fix Scarlet LysoBrite Green LysoTracker Green Mag-Fura-2 MiCy1 MitoLite Green FM MitoTracker Green Neuro DiO NeuroVue Jade NovaFluor Blue 510 Nuclear Yellow Oxazole Yellow Oxazole Yellow Homodimer PKH67 Pacific Green PhenoVue Fura-2 AM, Calcium Indicator PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain ReadiLink 405-508 STAR 440L Seta-385-NHS Spark Blue 515 Spark Violet 500 StarBright UltraViolet 510 Syto 9 T-Sapphire V500 violetFluor 500 VivaFix 408/512 VivaFix 498/521 YO-PRO-1 YOYO-1 Zombie Aqua Zombie Green eFluor 506 eFluor 506 Fix Viability eGFP mAzamiGreen BDY Lenalidomide