About Alexa Fluor 488

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Alexa Fluor™ 488 (AF488, Alexa 488) has an excitation peak at 488 nm and an emission peak at 496 nm, and is considered a high-performance alternative to FITC. Alexa 488 is one of the most popular Alexa Fluor™ dyes and is widely used in Fluorescence Microscopy, flow cytometry, and for staining low expression markers. It is bright, highly photostable, resistant to pH changes, and less susceptible to photobleaching. Alexa 488 and is similar in size, brightness and application to DyLight™ 488, iFluor® 488 and CF®488A.

Alternative Dyes to Alexa Fluor 488

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STAR 488 MaxLight 490 Chromeo 488 CoraLite Plus 488 VRDye 490 Fluorescein Oregon Green 488 Rhodamine 110 ATTO 488 CF488A Chromeo 505 FITC KIRAVIA Blue 520 NL 493 Oyster 488 PromoFluor 488P SureLight 488

Dyes similar to Alexa Fluor 488 based on emission

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5-CFDA 5-FAM ATTO 488 Acridine Orange Aldefluor Brilliant Blue 515 BCECF (525em) pH9.0 BCECF BCECF (Red em) pH5.2 BCECF AM BDY FL SE BDY FL-X SE BODIPY 500/510 BactoView Live Green Brilliant Violet 510 CFSE CF440 CF488A CF500 CPN510 Green Calcein Calcein AM Calcein Green Calcium Green Carboxyfluorescein CellBrite Fix 488 CellBrite Steady 488 CellROX Green CellTrace Oregon Green CellTracker Violet Chromeo 488 Chromeo 505 Citrine FITC Plus CoraLite Plus 488 CytoTrace Green CMFDA CytoTrack Green DCF DMAO DyLight 488 EFLUXX-ID Green ExoBrite 490/515 EV Membrane Stain FDG FITC FLUOFORTE FVS520 Flash Phalloidin Green 488 Fluo-3 Fluo-3 AM Fluo-4 Fluo-4 AM Fluorescein Fura-2 (Ca2+bound) Fura-2 (Ca2+free) GFP GelGreen Ghost Dye Blue 516 HiLyte 488 KIRAVIA Blue 520 Kaede (green) KikGR1 (green) Krome Orange Live-or-Dye 488/515 Live/Dead Fix Aqua Live/Dead Fix Green Live/Dead Fix Scarlet LysoBrite Green LysoTracker Green LysoView 488 Mag-Fluo-4 MagDot 525 MaxLight 490 MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green EX488 MitoLite Green FM MitoView Green MultiDot 525 NL 493 NovaFluor Blue 510 NucSpot Live 488 Oregon Green 488 Oregon Green 488 BAPTA Oregon Green 514 Oyster 488 PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluo-4 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Fluor 488 Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P Qdot 525 ROS-ID Green ReadiLink 492-516 Rhodamine 110 Rhodamine Green STAR 440L STAR 440SXP STAR 488 STAR GREEN SYBR Green Seta-385-NHS Spark Blue 515 Spectrum Green StarBright UltraViolet 510 StarBright Violet 515 SureLight 488 Syto 16 Sytox Green T-Sapphire TFAX 488 SE TFAX 488 TFP TSA Vivid 520 Thiazole Green Topaz VRDye 490 Venus Viafluor CFSE Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye Vio515 VioBright 515 VioBright FITC VioGreen VivaFix 408/512 VivaFix 498/521 YFP Zombie Aqua Zombie Green ZsGreen eFluor 514 Calcium Sensor eFluor 520 Fix Viability eYFP iFluor 488 mAmetrine BDY FL Staurosporine