About Aldefluor

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ALDeFluor™ is a non-immunological fluorescent reagent system for the detection of aldehyde dehydrogenase-bright (ALDHbr) in living cells. High expression of ALDH has been reported for normal and cancer stem and progenitor cells of various lineages, including hematopoietic, mammary, endothelial, mesenchymal and neural cells. It has an excitation peak at 488 nm and an emission peak at 512 nm. The ALDeFluor™ kit contains BODIPY-aminoacetaldehyde (BAAA), a fluorescent non-toxic substrate for ALDH, which freely diffuses into intact and viable cells. In the presence of ALDH, BAAA is converted into BODIPY-aminoacetate (BAA), a negatively charged product that is retained inside the cells. Intracellular accumulation of BAA leads to increased fluorescence, and these ALDH-bright (ALDHbr) cells can be analyzed with flow cytometry.

Dyes similar to Aldefluor based on emission

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5-CFDA 5-FAM ATTO 465 AccuClear Alexa Fluor 488 Brilliant Blue 515 BDY FL SE BDY FL-X SE BODIPY 500/510 BODIPY FL Brilliant Violet 510 CFSE CF440 CF488A CF500 CPN510 Green Calcein Calcein AM Calcein Green Carboxyfluorescein CellBrite Fix 488 CellBrite Green CellROX Green CellTracker Violet Chromeo 488 FITC Plus CoraLite Plus 488 Cy2 CytoTrace Green CMFDA DY-380XL DiO DyLight 488 EFLUXX-ID Green Emerald GFP ExoBrite 490/515 EV Membrane Stain FDG FLUOFORTE FVS510 FVS520 Flash Phalloidin Green 488 Fluo-4 Fluo-4 AM Fluorescein Fura-2 (Ca2+bound) Fura-2 (Ca2+free) GFP Ghost Dye Blue 516 Ghost Dye Violet 510 KIRAVIA Blue 520 Kaede (green) KikGR1 (green) Live-or-Dye 488/515 Live/Dead Fix Lime Live/Dead Fix Scarlet LysoBrite Green LysoTracker Green Mag-Fluo-4 Mag-Fura-2 MagDot 525 MaxLight 490 MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green FM MitoTracker Green MultiDot 525 NL 493 Neuro DiO NeuroVue Jade NovaFluor Blue 510 NucSpot Live 488 Nuclear Yellow Oregon Green 488 BAPTA Oxazole Yellow Oxazole Yellow Homodimer Oyster 488 PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fura-2 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Fluor 488 PromoFluor 488P ReadiLink 405-508 ReadiLink 492-516 Rhodamine 110 STAR 440L STAR 440SXP STAR GREEN SYBR Green Seta-385-NHS Spark Blue 515 Spectrum Green StarBright UltraViolet 510 StarBright Violet 515 SureLight 488 T-Sapphire TFAX 488 SE TFAX 488 TFP TSA Vivid 520 VRDye 490 Viafluor CFSE Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye Vio515 VioBright 515 VioGreen VivaFix 408/512 VivaFix 498/521 YO-PRO-1 YOYO-1 Zombie Aqua Zombie Green ZsGreen eFluor 506 eFluor 506 Fix Viability eFluor 514 Calcium Sensor eGFP iFluor 488 mAzamiGreen BDY FL Staurosporine BDY Lenalidomide