Qdot 525 Dye Profile

Invitrogen/Thermo Fisher Scientific™

320-405

Laser Line

515/30

Common Filter

300

Excitation Max

525

Emission Max

About Qdot 525


Qdot® 525 from Thermo Fisher Scientific is an inorganic nanocrystal with a surface functionalized to allow antibodies and biomolecules to be cross-linked. It is most efficiently excited between 320-405 nm but is capable of excitation across every laser less than its emission peak. It is significantly brighter and more photostable than traditional organic fluorophores, making it still useful for fluorescence microscopy, however its size and solubility issues make it a challenge to use in flow cytometry. In spectral flow cytometry it may still prove useful due to its very unique spectral characteristics



Spectra


Alternative Dyes to Qdot 525

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AmCyan Krome Orange Oregon Green 488 Pacific Green ATTO 465 FITC V500


Dyes similar to Qdot 525 based on emission

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ATTO 488 Acridine Orange Alexa Fluor 488 BCECF (525em) pH9.0 BCECF BCECF (Red em) pH5.2 BCECF AM BODIPY 500/510 BactoView Live Green CFSE CF503R Calcium Green CellBrite Steady 488 CellROX Green CellTrace Oregon Green CellTracker Violet Chromeo 488 Chromeo 505 Citrine FITC Plus CytoTrack Green DCF DMAO DyLight 488 EvaGreen EvaGreen Plus ExoBrite 490/515 EV Membrane Stain FITC Flash Phalloidin Green 488 Fluo-3 Fluo-3 AM Fluo-4 GOLGI-ID Green GelGreen HiLyte 488 KIRAVIA Blue 520 Kaede (green) KikGR1 (green) Krome Orange LIVE 510 Live-or-Dye 488/515 Live/Dead Fix Aqua Live/Dead Fix Green LysoView 488 Mag-Fluo-4 MagDot 525 MemBrite Fix 488/515 MitoLite Green EX488 MitoView Green MultiDot 525 Newport Green DCF NovaFluor Blue 530 NucSpot Live 488 NucView 488 Nuclear Green Nuclear-ID Green Oregon Green 488 Oregon Green 488 BAPTA Oregon Green 514 Oyster 488 PhenoVue Fluo-4 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P ROS-ID Green ReadiLink 492-516 Rhodamine 110 Rhodamine 123 Rhodamine Green Riboflavin STAR 488 STAR 512 STAR GREEN SYBR Green Spectrum Green SureLight 488 Syto 16 Sytox Green TFAX 488 SE TFAX 488 TFP TO-Pro 1 TOTO-1 TSA Vivid 520 Thiazole Green Thiazole Orange Thiazole Orange Homodimer Thiazole Orange Iodide Topaz Venus ViaFluor 488 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye VioBright FITC VioGreen Vybrant DyeCycle Green YFP ZsGreen eFluor 520 Fix Viability eYFP iFluor 488 mAmetrine mCitrine pHrodo Green BDY FL Staurosporine