About BCECF AM

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BCECF-AM is a cell-permeant pH indicator in its acetoxymethylester (AM) form with a pKa (6.97). This form of the pH indicator passively crosses the intact, live cell membrane where the AM ester moeity is cleaved by instracellular esterases, retaining the probe inside. At low pH, the reagent is weakly fluorescent but increases in intensity with increasing pH. This probe can be used ratiometrically as the excitation spectrum shifts between 439 nm and 505 nm, pH depending. This probe has, therefor, a primary excitation peak at 505 nm and a secondary peak at 439 nm with an emission peak that remains constant at 524 nm.

Dyes similar to BCECF AM based on emission

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5-FAM ATTO 488 Acridine Orange Alexa Fluor 488 Brilliant Blue 515 BCECF (525em) pH9.0 BCECF BCECF (Red em) pH5.2 BODIPY 500/510 BactoView Live Green CFSE CF440 CF488A CF503R Calcium Green Carboxyfluorescein CellBrite Steady 488 CellROX Green CellTrace Oregon Green CellTracker Violet Chromeo 488 Chromeo 505 Citrine FITC Plus CoraLite Plus 488 CytoTrack Green DCF DMAO DyLight 488 EvaGreen EvaGreen Plus ExoBrite 490/515 EV Membrane Stain FDG FITC FLUOFORTE FVS520 Flash Phalloidin Green 488 Fluo-3 Fluo-3 AM Fluo-4 Fluo-4 AM GOLGI-ID Green GelGreen Ghost Dye Blue 516 HiLyte 488 KIRAVIA Blue 520 Kaede (green) KikGR1 (green) Krome Orange LIVE 510 Live-or-Dye 488/515 Live/Dead Fix Aqua Live/Dead Fix Green LysoView 488 Mag-Fluo-4 MagDot 525 MaxLight 490 MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green EX488 MitoView Green MultiDot 525 Newport Green DCF NovaFluor Blue 530 NucSpot Live 488 NucView 488 Nuclear Green Nuclear-ID Green Oregon Green 488 Oregon Green 488 BAPTA Oregon Green 514 Oyster 488 PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluo-4 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator PhenoVue Fluor 488 Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P Qdot 525 ROS-ID Green ReadiLink 492-516 Rhodamine 110 Rhodamine 123 Rhodamine Green Riboflavin STAR 440SXP STAR 488 STAR 512 STAR GREEN SYBR Green Spectrum Green StarBright Violet 515 SureLight 488 Syto 16 Sytox Green TFAX 488 SE TFAX 488 TFP TO-Pro 1 TOTO-1 TSA Vivid 520 Thiazole Green Thiazole Orange Thiazole Orange Homodimer Thiazole Orange Iodide Topaz VRDye 490 Venus ViaFluor 488 Viafluor CFSE Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye VioBright FITC VioGreen YFP ZsGreen eFluor 520 Fix Viability eYFP iFluor 488 mAmetrine mCitrine pHrodo Green BDY FL Staurosporine