About Janelia Fluor 525

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Janelia Fluor® 525 was developed at the Janelia Campus of the Howard Hughes Medical Institute but is commercialized by other vendors. The Janelia Fluor®s family is unique in that the fluorophores are cell-permeable and are available in photoactivatable forms. These fluorophores were developed for super-resolution microscopy (STED, PALM and STORM) and live-cell microscopy in the HaloTag and SNAP-tag versions. Janelia Fluor® 525 has an excitation peak at 525 nm and an emission peak at 549 nm.

Alternative Dyes to Janelia Fluor 525

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ATTO 532

Dyes similar to Janelia Fluor 525 based on emission

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2-NBDG Alexa Fluor 430 Alexa Fluor 514 Alexa Fluor 532 ATTO 532 BODIPY R6G Calcein Orange Cascade Yellow CF405L CF514 CF517ST CF532 CoraFluor-1 CoraFluor-2 CPN550 Yellow DY-375XL EFLUXX-ID Gold ER-ID Green Ghost Dye Violet 540 iFluor 514 iFluor 532 Live/Dead Fix Yellow Live-or-Dye 405/545 Live-or-Dye 510/550 LysoBrite Orange LYSO-ID Green LysoSensor Yellow/Blue pH3.0 mBanana mHoneyDew MitoLite Orange EX405 NovaFluor Blue 555 Nuclear Orange NucSpot 470 Pacific Orange PhenoVue 512 Nucleic Acid Stain – complexed with nucleic acids Qdot 545 RealBlue 545 Rhodamine 6G Spark Blue 550 VivaFix 398/550 Vybrant DyeCycle Orange ExoBrite 515/540 True EV Membrane Stain XFD514 XFD532 cFluor V547 5(6)-Carboxyrhodamine RayBright LIVE V500 cFluor B548 StarBright UltraViolet 540 Spark PLUS B550 CoraFluor 2, amine reactive Lysosome Probe Orange StarBright Violet 535