Janelia Fluor 525 Dye Profile

Howard Hughes Medical Institute™

488/532

Laser Line

525/50

Common Filter

525

Excitation Max

549

Emission Max

122000

Extinction Coefficient

0.91

Quantun Efficiency

About Janelia Fluor 525


Janelia Fluor® 525 was developed at the Janelia Campus of the Howard Hughes Medical Institute but is commercialized by other vendors. The Janelia Fluor®s family is unique in that the fluorophores are cell-permeable and are available in photoactivatable forms. These fluorophores were developed for super-resolution microscopy (STED, PALM and STORM) and live-cell microscopy in the HaloTag and SNAP-tag versions. Janelia Fluor® 525 has an excitation peak at 525 nm and an emission peak at 549 nm.



Spectra