About NucSpot 470

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Biotium's NucSpot 470 is a cell membrane-impermeant dye that is fluorescent upon binding DNA. Since it is cell membrane-impermeant, it can be used to counterstain nuclei of fixed cells, or stain nuclei in dead cells. Other similar DNA binding dyes will often stain the nucleus and cytoplasm, while NucSpot 470 is specific to the nucleus, decreasing background signal. The more general NucSpot family of dyes has a few more members that have different functions compared to NucSpot 470. NucSpot Far-Red is an alternative to 7-AAD and is similarly used for staining cells with compromised cellular membranes, while NucSpot Live dyes are cell membrane-permeant DNA stains used for live cell staining. Check out the other members of this dye line in FluoroFinder's Dye Directory or on our Spectra Viewer.

Alternative Dyes to NucSpot 470

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7-AAD

Dyes similar to NucSpot 470 based on emission

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2-NBDG ATTO 532 Alexa Fluor 430 Alexa Fluor 514 Alexa Fluor 532 BODIPY R6G CF405L CF450 CF514 CF517ST CPN550 Yellow Calcein Orange Cascade Yellow CoraFluor-1 CoraFluor-2 DY-375XL ER-ID Green Ghost Dye Violet 540 Janelia Fluor 525 LYSO-ID Green Live-or-Dye 405/545 Live-or-Dye 510/550 Live/Dead Fix Yellow LysoBrite Orange LysoSensor Yellow/Blue pH3.0 MitoLite Orange EX405 NBD NovaFluor Blue 555 Nuclear Orange Oxazole Gold Pacific Orange Qdot 545 ReadiLink 405-537 RealBlue 545 Rhodamine 6G Spark Blue 550 Spark Violet 538 VivaFix 398/550 Vybrant DyeCycle Orange iFluor 514 mBanana mHoneyDew