About Alexa Fluor 532

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Alexa Fluor™ 532 (AF532, Alexa 532) has an excitation peak at 532 nm and an emission peak at 554 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC, iFluor® 514 (ATT Bioquest) and iFluor® 546 (ATT Bioquest). Many flow cytometers are equipped with 532nm lasers, so while Alexa 532 can be used for flow cytometry, PE is more commonly used due to its brightness and spectral characteristics. This dye is commonly used for fluorescence microscopy applications.

Alternative Dyes to Alexa Fluor 532

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NovaFluor Blue 555 ATTO 532

Dyes similar to Alexa Fluor 532 based on emission

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ATTO 532 ATTO 542 ATTO Rho6G BPE Brilliant Ultraviolet 563 CF405L CF514 CF517ST CF532 CF543 CPN550 Yellow Calcein Orange Cascade Yellow Chromeo 546 CoraFluor-1 CoraFluor-2 Cyanine 3 SE CytoTrack Yellow DY-485XL EFLUXX-ID Gold ER-ID Green Janelia Fluor 525 LYSO-ID Green Live-or-Dye 405/545 Live-or-Dye 510/550 Live/Dead Fix Olive Live/Dead Fix Yellow LysoBrite Orange MemBrite Fix 543/560 MemBrite Fix 543/560 MitoLite Orange EX405 NovaFluor Blue 555 NucSpot 470 NucView 530 Nuclear Orange Pacific Orange PhenoVue 512 Nucleic Acid Stain – complexed with nucleic acids Pyronin Y Qdot 545 RealBlue 545 Rhodamine 6G Spark Blue 550 VivaFix 398/550 Vybrant DyeCycle Orange iFluor 514 iFluor 532 mBanana mHoneyDew mKO mKusabira-Orange2 mOrange