About Live/Dead Fix Yellow

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The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Yellow has an excitation peak at 400 nm and an emission peak at 550 nm.

Alternative Dyes to Live/Dead Fix Yellow

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FVS575V Ghost Dye Violet 540 VivaFix 398/550

Dyes similar to Live/Dead Fix Yellow based on emission

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ATTO 532 Alexa Fluor 430 Alexa Fluor 532 BODIPY R6G CF405L CF514 CF517ST CF532 CPN550 Yellow Calcein Orange Cascade Yellow CoraFluor-1 CoraFluor-2 CytoTrack Yellow DY-375XL EFLUXX-ID Gold ER-ID Green Ghost Dye Violet 540 Janelia Fluor 525 LYSO-ID Green Live-or-Dye 405/545 Live-or-Dye 510/550 LysoBrite Orange MitoLite Orange EX405 NovaFluor Blue 555 NucSpot 470 Nuclear Orange Pacific Orange PhenoVue 512 Nucleic Acid Stain – complexed with nucleic acids Qdot 545 RealBlue 545 Rhodamine 6G Spark Blue 550 VivaFix 398/550 Vybrant DyeCycle Orange iFluor 514 iFluor 532 mBanana mHoneyDew