About Live/Dead Fix Violet

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The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Violet has an excitation peak at 402 nm and an emission peak at 452 nm.

Alternative Dyes to Live/Dead Fix Violet

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Ghost Dye Violet 450 VivaFix 410/450 FVS450

Dyes similar to Live/Dead Fix Violet based on emission

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4-MUP BFP CF350 CF405M CF408 Calcein Blue Calcein Blue AM Calcein Violet AM CellTrace Violet CellVue Lavender Atlantic Blue DAPI DY-360XL DiFMU pH3.0 DiFMU pH9.0 ExoBrite 410/450 EV Membrane Stain FVS450 FxCycle Violet Ghost Dye Violet 450 Hoechst (33258) Live-or-Dye 350/448 Live-or-Dye 405/452 Marina Blue Nuclear Violet Nuclear-ID Blue Oxazole Blue Oxazole Blue Homodimer POPO-1 Pacific Blue PhenoVue DAPI Nuclear Stain PhenoVue Hoechst 33342 Nuclear Stain ReadiLink 405-454 Tag-It Violet TagBFP V450 ViaFluor 405 Viobility 405/452 Fixable Dye violetFluor 450 VioBlue VivaFix 410/450 Zombie UV eFluor 450 eFluor 455UV Fix Viability iFluor 350