About Calcein Blue AM

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Calcein Blue, AM, is a fluorogenic blue fluorescent molecule (ex 359/ em 446) that penetrates the membranes of live cells. Once inside the live cell, Calcein Blue, AM is hydrolyzed by intracellular esterases into the fluorescent Calcein Blue which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. Reagents that contain an acetoxymethyl ester (AM) or diacetate (DA) side chain, are useful indicators of cell health and vitality, especially when used together with a probe for cell membrane integrity like an impermeant nucleic acid stain or fixable live/dead probe.

Dyes similar to Calcein Blue AM based on emission

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4-MUP 7-Amino-4-methylcoumarin (AMC) AMCA Alexa Fluor 350 BFP CF350 CF405M CF408 Calcein Blue Calcein Violet AM CellBrite Blue CellBrite Blue CellTrace Violet Atlantic Blue CytoTrack Blue DiFMU pH3.0 DiFMU pH9.0 ExoBrite 410/450 EV Membrane Stain FVS450 Ghost Dye Violet 450 Hoechst (33258) Hoechst (34580) Live-or-Dye 350/448 Live-or-Dye 405/452 Live/Dead Fix Blue Live/Dead Fix Violet LysoSensor Yellow/Blue pH9.0 MitoView 405 Pacific Blue PhenoVue Hoechst 33342 Nuclear Stain ReadiLink 405-454 StarBright UltraViolet 445 Tag-It Violet TagBFP V450 ViaFluor 405 Viobility 405/452 Fixable Dye violetFluor 450 VioBlue VivaFix 353/442 VivaFix 410/450 Vybrant DyeCycle Violet Zombie UV eBFP eFluor 450 eFluor 450 Cell Proliferation eFluor 450 Fix Viability eFluor 455UV Fix Viability iFluor 350