Calcein Violet AM Dye Profile

405

Laser Line

450/50

Common Filter

399

Excitation Max

449

Emission Max

About Calcein Violet AM


Calcein Violet is a blue fluorescent molecule that penetrates the membranes of dead cells and is used to evaluate cell membrane integrity as well as esterase activity. Closely related is Calcein Violet AM, which due to the acetoxymethyl (AM) modification, can passively diffuse across the membranes of live cells. While inside the live cell, Calcein Violet AM is hydrolyzed by esterase into the fluorescent Calcein Violet which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. It has an excitation peak at 399 nm and an emission peak at 449 nm.



Spectra


Popular Calcein Violet AM Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
BioLegend
425203
FC Calcein Violet AM
All Species
N/A
IgG
10 x 25 μg
$211
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