About TLR8
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X. [provided by RefSeq, Jul 2008]

About Alexa Fluor 546
Alexa Fluor™ 546 (AF546, Alexa 546) has an excitation peak at 556 nm and an emission peak at 573 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC and iFluor® 555 (ATT Bioquest). Many flow cytometers are equipped with 532nm lasers, so while Alexa 546 can be used for flow cytometry, PE is more commonly used due to its brightness and spectral characteristics. This dye is commonly used for fluorescence microscopy applications.