About 7-AAD

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7-Aminoactinomycin D (7-AAD) is a red-emitting DNA intercalating fluorophore that binds to G-C rich regions of dead cells or cells with compromised membranes. It can be excited by the 488 nm, 532 nm and 561 nm lasers and collected using a 650 nm long-pass filter. 7-AAD has an excitation peak at 546 nm and an emission peak at 647 nm. NucSpot® Far-Red (Biotium) and Sytox AADvanced (Thermo Fisher Scientific) are poplular alternatives to 7-AAD due to reduced spillover characteristics. 7-AAD is primarily used for dead cell staining, as it is excluded from live cells with intact membranes, however due to the dysregulation of plasma membrane charge that happens with apoptosis, 7-AAD often is an indicator of late-stage apoptosis. This dye can be used in Fluorescence Microscopy or Flow Cytometry applications. This is a generic dye that can be found in many supplier's catalogs.

Dyes similar to 7-AAD based on emission

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ATTO Rho14 Alexa Fluor 633 Alexa Fluor 635 BDY 630-X SE BODIPY 630/650 Brilliant Violet 650 CF620R CF633 Calcein Deep Red CellTracker Deep Red CytoTrack Red E2-Crimson Helix NP NIR Janelia Fluor 635 LysoBrite NIR MITO-ID (Extracellular O2) MagDot 640 MagDot 655 MitoLite Deep Red FX660 MitoView 633 MultiDot 640 MultiDot 655 Nuclear-ID Red PE-Fire 640 Qdot 655 ReadiLink 633-655 SNARF 1 pH9.0 STAR 635P SuperBright 645 Syto 61 Sytox AADvanced Sytox Red TagRFP657 ViaKrome 638 eFluor 650NC eFluor 660 Fix Viability eVolve 655 iFluor 633 mNeptune mPlum