CD8b.2 Monoclonal / FITC / REA865
Product Details
| Description | Clone REA865 recognizes the beta chain of the mouse CD8 antigen, a single-pass type I membrane protein also known as lymphocyte antigen 3 (Ly-3.2 or Lyt-3.2). The CD8 molecule is composed of two distinct polypeptide chains that pair on the cell surface either as a CD8 alphaalpha homodimer or as a CD8 alphabeta heterodimer. These forms of the CD8 molecule are differentially expressed on functionally distinct CD8+ lymphocyte subsets. The CD8 alphabeta heterodimer is expressed on cytotoxic T cells and thymocytes. CD8b functions as a co-receptor for the T cell receptor (TCR). | Additional information: Clone REA865 displays negligible binding to Fc receptors. | |
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| Conjugate | FITC | |
| Clone | REA865 | |
| Target Species | Mouse | |
| Applications | FC | |
| Supplier | Miltenyi Biotec | |
| Catalog # | Sign in to view product details, citations, and spectra | |
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About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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Validation References
| PMID 8976201 | |
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| PMID 11114424 | |
| PMID 16356863 | |
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Reviews & Ratings
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