About CD35
This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The genome is polymorphic at this locus with allele-specific splice variants encoding different isoforms, based on the presence/absence of long homologous repeats (LHRs). The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in this gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus, sarcoidosis and Alzheimer's disease. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. [provided by RefSeq, May 2020]

About Brilliant Violet 480
Brilliant™ Violet 480 (BV480) is a green-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 525/40 bandpass filter. BV480 has an excitation peak at 436 nm and an emission peak at 478 nm, and provides an optimized alternative to BV510. Other dyes that are similar include StarBright Violet 475 (Bio-Rad) While BV480 can be detected on the same filter as BV510, its emmision profile reduces spillover into the BV605, BV650, and BV711 channels. Additionally BV480's excitation profile will reduce cross-laser excitation with the UV laser, resulting in less spillover into UV channels than BV510. These advantages make BV480 an optimal choice for flow cytometry panels using multiple reagents on Violet and/or UV lasers. This dye is part of the Brilliant™ Violet dye line. BV510 and BV480 are the only dyes in the Brilliant™ Violet dye family that are not tandem fluorophores based off the BV421 polymer core, but they have nearly the same maximum excitation.