P2X7R Monoclonal / FITC / REA875
Product Details
| Description | Clone REA875 recognizes the mouse P2X7R antigen, also known as P2RX7 or P2X7 (P2X purinoceptor 7), which is a receptor for ATP that acts as a ligand-gated ion channel. P2X7R is involved in different inflammatory, immune, neurologic, and musculoskeletal disorders and is able to modulate LPS-induced macrophage production of inflammatory mediators. P2X7R is found on cells of the immune system, e.g., mast cells, microglia, macrophages, T cells, and B cells, in uterine endometrium, retina, and in the central and peripheral nervous systems. | Additional information: Clone REA875 displays negligible binding to Fc receptors. | |
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| Conjugate | FITC | |
| Clone | REA875 | |
| Target Species | Mouse | |
| Applications | FC | |
| Supplier | Miltenyi Biotec | |
| Catalog # | Sign in to view product details, citations, and spectra | |
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About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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Validation References
| PMID 9849870 | |
|---|---|
| PMID 22349510 | |
| PMID 24857862 | |
| PMID 24928329 | |
| Additional Sources |
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Reviews & Ratings
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