CD34 / PerCP-Cy5.5 /
Product Details
| Description | The 581 antibody reacts with human CD34, an ~105 - 120 kDa type 1 transmembrane glycoprotein expressed on the surface of most human hematopoietic stem and progenitor cells (HSPCs) as well as on mesenchymal stem cells, embryonic fibroblasts, endothelial cells, neurons, and some tumor cell lines. CD34 is expressed only transiently during hematopoiesis, so the frequency of CD34+ cells is low in bone marrow or cord blood (~1 - 5%) and very low (~0.1 - 0.5%) in peripheral blood. CD34 is a marker used to identify and isolate HSPCs capable of cell engraftment. CD34 is thought to mediate attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells during early hematopoiesis, and to be involved in lymphocyte recruitment through binding to the ligands L- and E-selectin. Distinct epitope groups have been assigned to CD34 based on their sensitivity to enzymatic cleavage, with the 581 antibody recognizing a class III epitope (resistant to neuraminidase and O-glycoprotease). | |
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| Conjugate | PerCP-Cy5.5 | |
| Clone | ||
| Target Species | Human, Monkey | |
| Applications | FC | |
| Supplier | Abbexa | |
| Catalog # | Sign in to view product details, citations, and spectra | |
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About CD34
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
About PerCP-Cy5.5
PerCP-Cyanine® 5.5 (PerCP-Cy5.5) is a red-emitting tandem fluorophore that was originally designed to make the PerCP fluorophore more stable and increase signal intensity. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-Cy5.5 has an excitation peak at 482 nm and an emission peak at 695 nm. There are superior alternatives to PerCP and PerCP-Cy5.5 including BB700, NovaFluor Blue 690 or PerCP-eFluor™ 710
PerCP-Cyanine® 5.5 (PerCP-Cy5.5) is a red-emitting tandem fluorophore that was originally designed to make the PerCP fluorophore more stable and increase signal intensity. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-Cy5.5 has an excitation peak at 482 nm and an emission peak at 695 nm. There are superior alternatives to PerCP and PerCP-Cy5.5 including BB700, NovaFluor Blue 690 or PerCP-eFluor™ 710
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