CD45RC / FITC /

Product Details

Description	This antibody reacts with CD45RC on pre-B lymphocytes,B cells,CD8+ T suppressor/cytotoxic cells,and a subset of CD4+ T helper (Th)lymphocytes. It weakly reacts with thymocytes.4 CD45RC is a high-molecular-weight isoform of CD45 (Leukocyte Common Antigen);its level of expression distinguishes subpopulations of CD4 +T cells with Th1-like and Th2-like effector functions. Levels of expression of CD45RC have also been reported to distinguish resting from activated T cells at various stages of maturation. CD45 is a member of the Protein Tyrosine Phosphatase (PTP)family:Its intracellular (COOH-terminal)region contains two PTP catalytic domains,and the extracellular region is highly variable due to alternative splicing of exons 4,5,and 6 (designated A,B,and C,respectively),plus differing levels of glycosylation. The CD45 isoforms detected in the rat are cell type-,maturation-,and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.
Conjugate	FITC
Clone
Target Species	Human
Applications	FC
Supplier	Abbexa
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About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

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