IgG2a Secondary Antibody / CF405S /

Product Details

Description	Goat anti-mouse IgG2a isotype-specific secondary antibody labeled with CF®405S dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Blue fluorescent CF®405S dye has excitation/emission at 404/431 nm. Note: Conjugates of blue fluorescent dyes like CF®350, CF®405S, and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.The conjugate is prepared from affinity-purified antibodies that react with Fc portion of the heavy chain of mouse IgG2a. To minimize cross reactivity, the antibodies are cross-adsorbed against other mouse IgG subclasses (IgG1, IgG2b, IgG3), and human, bovine and rabbit serum proteins.
Conjugate	CF405S
Clone
Target Species	Mouse
Applications	IF, IHC-P, WB, IHC-Fr
Supplier	Biotium
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	Antigen
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	Isotype

About IgG2a
Predicted to enable antigen binding activity and immunoglobulin receptor binding activity. Predicted to be involved in several processes, including activation of immune response; defense response to other organism; and phagocytosis. Located in extracellular space. [provided by Alliance of Genome Resources, Apr 2022]

About CF405S
CF®405S from Biotium is a fluorophore with an excitation peak at 404 nm and an emission peak at 431 nm. It is spectrally similar to Alexa Fluor™ 405. It can be use in fluorescence microscopy, specifically its been validated in Structured Illumination (SIM), and flow cytometry. However, in flow cytometry, superior alternatives are Brilliant™ Violet 421, SuperNova v428 and StarBright Violet 440.

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