CD68 Monoclonal / PerCP-Vio700 / REA835

Product Details
Description Clone REA835 recognizes mouse CD68 antigen, also known as macrosialin, which is a heavily glycosylated type I transmembrane protein of the lysosomal-associated membrane protein (LAMP) family. It is expressed on tissue macrophages, Langerhans cells, and at a low density on dendritic cells. Murine CD68 is predominantly intracellular but it is reported that 10–15% of CD68 is expressed on the cell surface as well. Macrosialin functions as a macrophage receptor for oxidized low density lipoprotein. | Additional information: Clone REA835 displays negligible binding to Fc receptors.
Conjugate PerCP-Vio700
Clone REA835
Target Species Mouse
Applications FC
Supplier Miltenyi Biotec
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About CD68
This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
About PerCP-Vio700
PerCP-Vio® 770 is a far-red-emitting tandem fluorophore that combines PerCP and a Vio®770 dye. The donor, PerCP, can be excited by the 488 nm blue laser and transfers energy to the acceptor, Vio®770, which emits light that can be captured with a 780/60 nm bandpass filter. APC-Vio®770 has an excitation peak at 482 nm and an emission peak at 704 nm, and is spectrally similar to PerCP-Cy5.5 and PerCP-eF710. PerCP-Vio®770 is known to be very bright and photostable, as well as being stable when treated with fixatives. These favorable characteristics that make it useful in multiple different applications such as flow cytometry and Fluorescence Microscopy. The large stokes shift can be advantageous when trying to fit more colors into a multicolor panel. The Vio® dye family are products of Miltenyi Biotec, with many antibody conjugates designed and validated for flow cytometry.
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