CD45R (B220) Monoclonal / PerCP-Vio700 / REA755

Product Details

Description	Clone REA755 recognizes the mouse CD45R (B220) antigen, a B lineage-specific surface molecule, which is expressed on B lymphocytes throughout their development from early pro–B stages onwards and is down-regulated upon terminal differentiation to plasma cells. Apart from B cells, CD45R is expressed on a small subset of dendritic cells (plasmacytoid dendritic cells). CD45R is absent in thymus but reported to be present on apoptotic thymocytes. Clone REA755 specifically recognizes the exon A–restricted isoform of mouse CD45. \| Additional information: Clone REA755 displays negligible binding to Fc receptors.
Conjugate	PerCP-Vio700
Clone	REA755
Target Species	Mouse
Applications	FC
Supplier	Miltenyi Biotec
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About PerCP-Vio700
PerCP-Vio® 770 is a far-red-emitting tandem fluorophore that combines PerCP and a Vio®770 dye. The donor, PerCP, can be excited by the 488 nm blue laser and transfers energy to the acceptor, Vio®770, which emits light that can be captured with a 780/60 nm bandpass filter. APC-Vio®770 has an excitation peak at 482 nm and an emission peak at 704 nm, and is spectrally similar to PerCP-Cy5.5 and PerCP-eF710. PerCP-Vio®770 is known to be very bright and photostable, as well as being stable when treated with fixatives. These favorable characteristics that make it useful in multiple different applications such as flow cytometry and Fluorescence Microscopy. The large stokes shift can be advantageous when trying to fit more colors into a multicolor panel. The Vio® dye family are products of Miltenyi Biotec, with many antibody conjugates designed and validated for flow cytometry.

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