CD43 Monoclonal / PerCP-Vio700 / REA833

Product Details
Description Clone REA833 recognizes the human CD43 antigen, also known as leukosialin, a cell surface sialoglycoprotein that is involved in cell adhesion, differentiation, and activation. The CD43 antigen is expressed on most leukocytes, i.e., T cells, NK cells, granulocytes, monocytes, and macrophages, on hematopoietic stem cells and on platelets, but not on erythrocytes. Among B cells, CD43 is expressed on activated B cells and plasma cells but not on resting B cells, e.g., naive B cells. In bone marrow, CD43 is found on pro–B cells but is down-regulated during transition to the pre–B cell stage. CD43 is expressed on different myeloid and lymphoid tumors, including some B cell malignancies. | Additional information: Clone REA833 displays negligible binding to Fc receptors.
Conjugate PerCP-Vio700
Clone REA833
Target Species Human
Applications FC
Supplier Miltenyi Biotec
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About CD43
This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017]
About PerCP-Vio700
PerCP-Vio® 770 is a far-red-emitting tandem fluorophore that combines PerCP and a Vio®770 dye. The donor, PerCP, can be excited by the 488 nm blue laser and transfers energy to the acceptor, Vio®770, which emits light that can be captured with a 780/60 nm bandpass filter. APC-Vio®770 has an excitation peak at 482 nm and an emission peak at 704 nm, and is spectrally similar to PerCP-Cy5.5 and PerCP-eF710. PerCP-Vio®770 is known to be very bright and photostable, as well as being stable when treated with fixatives. These favorable characteristics that make it useful in multiple different applications such as flow cytometry and Fluorescence Microscopy. The large stokes shift can be advantageous when trying to fit more colors into a multicolor panel. The Vio® dye family are products of Miltenyi Biotec, with many antibody conjugates designed and validated for flow cytometry.
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