p53 (pS15) Monoclonal / PE / REA825
Product Details
Description | Clone REA825 recognizes the human cellular tumor antigen p53 phosphorylated at serine 15 (pS15). The p53 protein is an important regulator of cell growth and, thus, functions as a tumor suppressor in many tumor types. The p53 tumor suppressor gene is altered or deleted in most solid human tumors. It is tightly regulated and plays a critical role in the cellular response to environmental stress. Upon a cell's exposure to stress, such as hypoxia or genomic damage, the p53 protein is expressed at high levels and is post-translationally modified. These modifications, which include phosphorylation, acetylation, and glycosylation, occur rapidly and lead to the activation of p53, resulting in either G1 growth arrest or programmed cell death. Following DNA damage, p53 is phosphorylated on serine residues 15, 20, 33, and 37 within the amino-terminal domain. These phosphorylation events are thought to play a key role in regulating p53 stability and activity. | Additional information: Clone REA825 displays negligible binding to Fc receptors. | |
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Conjugate | PE | |
Clone | REA825 | |
Target Species | Human | |
Applications | FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC | |
Supplier | Miltenyi Biotec | |
Catalog # | Sign in to view product details, citations, and spectra | |
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About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
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Validation References
PMID 1406679 | |
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PMID 4006916 | |
PMID 16501611 | |
Additional Sources |
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