IL-17F Monoclonal / FITC / REA666

Product Details

Description	Clone REA666 recognizes the mouse interleukin 17F (IL-17F) antigen, a cytokine which is expressed by a subset of activated T cells in the lamina propria. IL-17F belongs to the IL-17 gene family, which consists of six members (IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, and IL-17F). All members have a similar protein structure, with four highly conserved cysteine residues critical to their 3-dimensional shape. The isoforms of IL-17F 1 and 2 (ML-1) have the highest homology to IL-17A. It has been reported, that IL-17–producing T lymphocytes comprise a distinct lineage of proinflammatory T helper cells, termed Tʜ17 cells, that are major contributors to autoimmune disease. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of Ag-induced pulmonary inflammation. \| Additional information: Clone REA666 displays negligible binding to Fc receptors. \|
Conjugate	FITC
Clone	REA666
Target Species	Mouse
Applications	FC
Supplier	Miltenyi Biotec
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About IL-17F
The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. [provided by RefSeq, Jul 2008]

About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

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