Fc epsilon R1 alpha Monoclonal / FITC / REA758

Product Details
Description Clone REA758 recognizes the human FcepsilonRIalpha, the alpha subunit of the human high-affinity Fc receptor for IgE. The receptor is composed of one alpha-, one beta-, and two disulphide-linked gamma-chains, of which the alpha subunit binds IgE1. FcepsilonRIalpha is expressed on mast and basophil cells, is upregulated in the presence of IgE, and is found on a subset of blood dendritic cells. The FcepsilonRI complex plays an important role in triggering IgE-mediated allergic reactions. | Additional information: Clone REA758 displays negligible binding to Fc receptors.
Conjugate FITC
Clone REA758
Target Species Human
Applications FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier Miltenyi Biotec
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About Fc epsilon R1 alpha
The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Aug 2011]
About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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