Streptavidin / Alexa Fluor 546 / N/A

Product Details

Description	Alexa Fluor® 546 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.
Conjugate	Alexa Fluor 546
Clone	N/A
Target Species	Biotin
Applications	FC, IF
Supplier	Thermo Fisher Scientific
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About Alexa Fluor 546
Alexa Fluor™ 546 (AF546, Alexa 546) has an excitation peak at 556 nm and an emission peak at 573 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC and iFluor® 555 (ATT Bioquest). Many flow cytometers are equipped with 532nm lasers, so while Alexa 546 can be used for flow cytometry, PE is more commonly used due to its brightness and spectral characteristics. This dye is commonly used for fluorescence microscopy applications.

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