About TCR gamma/delta
T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor gamma locus. The gamma locus includes V (variable), J (joining), and C (constant) segments. During T cell development, the gamma chain is synthesized by a recombination event at the DNA level joining a V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random addition of nucleotides by terminal deoxynucleotidyltransferase. Several V segments of the gamma locus are known to be incapable of encoding a protein and are considered pseudogenes. Somatic rearrangement of the gamma locus has been observed in T cells derived from patients with T cell leukemia and ataxia telangiectasia. [provided by RefSeq, Jul 2008]

About Brilliant Ultraviolet 395
Brilliant™ UltraViolet 395 (BUV395) is a UV-emitting base polymer dye that can be excited by the 355 nm ultraViolet laser and collected using a 379/28 bandpass filter. BUV395 has an excitation peak at 348 nm and an emission peak at 395. BUV395's fairly unique excitation and emission spectrum causes relatively little spillover into other common detectors and conflicts with very few other commonly used fluorophores. An alternative to BUV395 for flow cytometry is StarBright UltraViolet 400. This dye is part of the Brilliant™ UltraViolet dye family, developed and sold by BD Biosciences. These dyes were optimized for use with multicolor flow cytometry. BUV395 forms the backbone of the family, as all other members are tandems that use it as the acceptor, thus making them excitable by the same 355 nm UV laser.