About CD4
This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, May 2020]

About Brilliant Violet 480
Brilliant™ Violet 480 (BV480) is a green-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 525/40 bandpass filter. BV480 has an excitation peak at 436 nm and an emission peak at 478 nm, and provides an optimized alternative to BV510. Other dyes that are similar include StarBright Violet 475 (Bio-Rad) While BV480 can be detected on the same filter as BV510, its emmision profile reduces spillover into the BV605, BV650, and BV711 channels. Additionally BV480's excitation profile will reduce cross-laser excitation with the UV laser, resulting in less spillover into UV channels than BV510. These advantages make BV480 an optimal choice for flow cytometry panels using multiple reagents on Violet and/or UV lasers. This dye is part of the Brilliant™ Violet dye line. BV510 and BV480 are the only dyes in the Brilliant™ Violet dye family that are not tandem fluorophores based off the BV421 polymer core, but they have nearly the same maximum excitation.