CD16 / Alexa Fluor 700 / CB16

Product Details

Description	It recognizes CD16 (Fcgamma RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fcgamma RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fcgamma RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.
Conjugate	Alexa Fluor 700
Clone	CB16
Target Species	Human
Applications	FC
Supplier	Novus Biologicals
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About Alexa Fluor 700
Alexa Fluor™ 700 (AF700, Alexa 700) has an excitation peak at 702 nm and an emission peak at 723 nm, and is spectrally similar to iFluor® 680 (ATT Bioquest) and iFluor® 700 (ATT Bioquest). Alexa 700 is commonly used in Flow Cytometry and is similar in size, brightness and application to iFluor® 700 and Vio® R720.

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