Fura Red / Fura Red AM (Ca2+bound), Fura Red AM (Ca2+free) / N/A
Product Details
| Description | Fura Red, AM is a visible light—excitable fura-2 analog that offers unique possibilities for ratiometric measurement of Ca2+ in single cells by microphotometry, imaging or flow cytometry when used with single excitation, green-fluorescent calcium indicators. This acetoxymethyl (AM) ester form is useful for noninvasive intracellular loading and is also available in special packaging (F-3021). | |
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| Conjugate | Fura Red AM (Ca2+bound), Fura Red AM (Ca2+free) | |
| Clone | N/A | |
| Target Species | All Species | |
| Applications | FC, IF | |
| Supplier | Thermo Fisher Scientific | |
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About Fura Red AM (Ca2+free)
Fura Red, AM is an intracellular calcium indicator. When Fura Red is hydrolyzed intracellularly and binds cytoplasmic Ca2+, the excitation efficiency of the probe at 405 and 488nm decreases which diminishes the emission of the probe at ~660nm. It is commonly used with Fluo-4, AM which exhibits increased intensity when bound to ratiometrically assess calcium concentration at 488nm excitation.
Fura Red, AM is an intracellular calcium indicator. When Fura Red is hydrolyzed intracellularly and binds cytoplasmic Ca2+, the excitation efficiency of the probe at 405 and 488nm decreases which diminishes the emission of the probe at ~660nm. It is commonly used with Fluo-4, AM which exhibits increased intensity when bound to ratiometrically assess calcium concentration at 488nm excitation.
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2 Fura Red antibodies available with over 1 conjugates.
