5-CFDA / 5-CFDA / N/A

Product Details

Description	5-CFDA, AM is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. The electronically neutral AM ester of CFDA can be loaded into cells at lower concentrations than CFDA. Upon hydrolysis by intracellular esterases, this AM ester also yields carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells.
Conjugate	5-CFDA
Clone	N/A
Target Species	All Species
Applications	IF
Supplier	Thermo Fisher Scientific
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About 5-CFDA
5-CFDA is a single isomer form of the fluorogenic carboxyfluorescein diacetate (CFDA) often used in live-cell applications to assess cell health and viability. CFDA can also be used as a short-term cell tracker. For long term cell tracking or proliferation assays use instead the CFDA-SE (sometimes errantly abbreviated CFSE) reagent which loads the cell identically but is retained once internalized by the subsequent conjugation of the succinimidyl ester moiety to intracellular amine-containing proteins and peptides.

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