Fetal Hemoglobin Monoclonal / PE / REA533

Product Details

Description	Clone REA533 recognizes the human fetal hemoglobin (HbF) antigen, a form of hemoglobin present during fetal development. The hemoglobin molecule is a tetramer composed of two subunits of alpha-like globin peptide chains and two subunits of the beta-like globin peptides, along with heme moieties necessary for this molecule´s oxygen-carrying capacity. When production of the first enucleated definitive erythrocytes commences from stem and progenitor cells in the fetal liver, the predominant beta-like globin molecule produced is gamma-globin. The gamma-globin chains combine with adult alpha-globin chains into a stable tetramer forming HbF. Shortly after the time of birth there is a switch from predominant expression of HbF to adult hemoglobin (HbA), which is mediated by a transcriptional switch in definitive erythroid progenitors from gamma- to beta-globin. The synthesis of HbF is normally reduced to very low levels of less than 0.6% of the total hemoglobin in adults. HbF levels may be increased in adults in a number of inherited and acquired disorders. The clinical significance of these conditions with raised HbF relates to their interaction in disorders such as sickle cell disease and beta-thalassemia in which raised levels of HbF can lead to considerable amelioration of disease severity. \| Additional information: Clone REA533 displays negligible binding to Fc receptors.
Conjugate	PE
Clone	REA533
Target Species	Human
Applications	FC
Supplier	Miltenyi Biotec
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About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.

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