PLK-1 (pT210) Monoclonal / PE / REA530

Product Details

Description	Clone REA530 recognizes the human polo-like kinase (PLK1) antigen phosphorylated at threonine 210 (pT210). PLK1 is a key regulator of several important cell-cycle–associated processes, such as centrosome maturation, spindle assembly, sister chromatid cohesion, cytokinesis, and recovery from a DNA-damage–induced arrest. Expression of PLK1 is highly cell cycle regulated; its expression is first induced in G2 and peaks during the early stages of mitosis. PLK1 has been described to be recruited to the centrosomes in G2, as well as to spindle poles and kinetochores during mitosis. Activation of PLK1 is dependent on phosphorylation of a conserved threonine residue (T210) in the T-loop of its kinase domain. Phosphorylation of this residue starts in G2, when PLK1 activity gradually increases until it reaches its full activity when cells enter mitosis. The initial phosphorylation of T210 in G2 is mediated by the kinase Aurora-A, in concert with its cofactor Bora. Since PLK1 is highly expressed in several carcinomas, and expression is inversely correlated with the survival rate of patients in non-small cell lung, head and neck, and esophageal cancer, PLK1 is recognized as a valid prognostic marker. \| Additional information: Clone REA530 displays negligible binding to Fc receptors.
Conjugate	PE
Clone	REA530
Target Species	Human
Applications	FC
Supplier	Miltenyi Biotec
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About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.

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