MRP1 Monoclonal / FITC / REA481

Product Details

Description	Clone REA481 recognizes an intracellular epitope of the human multidrug resistance–associated protein 1 (MRP1) antigen, a 190 kDa multi-pass membrane protein also known as ATP-binding cassette sub-family C member 1 (ABCC1) or leukotriene C(4) transporter. MRP1 was initially identified because of its ability to confer multidrug resistance in lung cancer cells. It plays a role in protecting certain tissues from xenobiotic insults and that it mediates the ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs, and other xenobiotics from the cytoplasm. MRP1 also plays a role in the cellular efflux of the reduced and oxidized forms of glutathione and thus contributes to the many physiological and pathophysiological processes influenced by these small peptides, including oxidative stress. \| Additional information: Clone REA481 displays negligible binding to Fc receptors. \|
Conjugate	FITC
Clone	REA481
Target Species	Human
Applications	FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier	Miltenyi Biotec
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About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

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