E-Selectin Monoclonal / PE / REA280

Product Details

Description	Clone REA280 recognizes the CD62 antigen-like family member E (CD62E) antigen, a 115 kDa single-pass type I membrane protein which is also known as E-selectin, endothelial-leukocyte adhesion molecule 1 (ELAM-1), or leukocyte-endothelial cell adhesion molecule 2 (LECAM2). CD62E is a cell adhesion molecule highly expressed only on endothelial cells activated by cytokines. It is not stored in the cell and has to be transcribed, translated, and transported to the cell surface. The production of CD62E is stimulated by the expression of CD62P which is stimulated by tumor necrosis factor alpha, and it can also be stimulated by interleukin-1 and lipopolysaccharide. CD62E recognizes and binds to sialylated carbohydrates present on the surface proteins of certain leukocytes. CD62E ligands are expressed by neutrophils, monocytes, eosinophils, memory-effector T-like lymphocytes, and natural killer cells. Each of these cell types is found in acute and chronic inflammatory sites in association with expression of CD62E, thus implicating CD62E in the recruitment of these cells to such inflammatory sites. \| Additional information: Clone REA280 displays negligible binding to Fc receptors.
Conjugate	PE
Clone	REA280
Target Species	Human
Applications	FC
Supplier	Miltenyi Biotec
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About E-Selectin
The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008]

About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.

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