CFSE / CFSE / N/A

Product Details

Description	CFSE is a reagent useful for cell tracking, proliferation studies, and cell motility studies. After passively diffusing into cells, it is converted to a fluorescent carboxyfluorescin molecule as its acetate groups are cleaved by intracellular esterases. The fluorescent CFSE is retained inside the cell for extended periods, because the molecules forms stable covalent crosslinks with the intracellular proteins. At each cell division, the intensity of the CFSE fluorescence is halved. The peak excitation of the molecule is 494 nm and the peak emission is 521 nm and it may be used with standard fixatives containing formaldehyde and permeabilization buffers that contain saponin.
Conjugate	CFSE
Clone	N/A
Target Species	All Species
Applications	FC
Supplier	BioGems
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About CFSE
Carboxyfluorescein diacetate succinimidyl ester (CFSE) is a blue-emitting amine-reactive dye that can be excited by the 405 nm Violet laser and captured using a 530/43 bandpass filter. It has excitation peak at 494 nm and and an emission peak at 521 nm. CFSE is most commonly used for in vivo cell tracking and proliferation studies. When a cell reproduces, the dye is distributed evenly to daughter cells , with fluorescence intensity halving with each successive passage. This is a generic dye and it can be found in kits from various suppliers.

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