Indo-Lo (Ca2+free) / Indo-Lo (Ca2+free) / N/A

Product Details

Description	Indo-1 acetomethoxy (AM) is a popular UV-excitable calcium indicator, similar to Fura-2. In contrast to Fura-2, Indo-1 has a dual emission peak. The main emission peak in calcium-free solution is 475nm while in the presence of calcium the emission is shifted to 400nm. Indo-1 AM is a membrane permeable probe and can be easily loaded into cells by passive incubation. The probe is widely used in flow cytometry. For this application, it is critical to have an instrument with an argon laser tuned to UV or a heliumcadmium laser available. Wavelength Maxima: Excitation 346nm, Emission 475nm
Conjugate	Indo-Lo (Ca2+free)
Clone	N/A
Target Species	All Species
Applications	FC
Supplier	Enzo Life Sciences
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About Indo-Lo (Ca2+free)
Indo-1, AM is a ratiometric calcium indicator optimally excited by a 355nm UV laser or excitation source. After Indo-1, AM is loaded into live cells, it is hydrolyzed and available to bind cytoplasmic Ca2+. Probe bound to Ca2+ emit at ~400nm, while probe unbound to Ca2+ emits at ~475nm. Because of these unique spectral characteristics, it's the preferred calcium indicator for flow cytometry and is often frequently used in fluorescence live-cell microscopy.

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