Calcein Blue AM / Calcein Blue AM / N/A

Product Details
Description Calcein Blue has similar calcium-binding properties as calcein. Like calcein, it is typically used for metallochromic titration of metal ions with EDTA and demonstrates enhanced fluorescence upon binding Ca (II), Zn (II) or La (II). Binding of Mn (II), Fe (II), Co (II), Ni (II) and Cu (II) results in a reduction in fluorescence. In addition, the probe is also used as a reference standard for calcein AM. The probe also finds application in cell-based assays for measuring mineralized-tissue formation by cultured osteoblastic cells. Wavelength Maxima: Excitation 360nm, Emission 445nm
Conjugate Calcein Blue AM
Clone N/A
Target Species All Species
Applications FC
Supplier Enzo Life Sciences
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About Calcein Blue AM
Calcein Blue, AM, is a fluorogenic blue fluorescent molecule (ex 359/ em 446) that penetrates the membranes of live cells. Once inside the live cell, Calcein Blue, AM is hydrolyzed by intracellular esterases into the fluorescent Calcein Blue which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. Reagents that contain an acetoxymethyl ester (AM) or diacetate (DA) side chain, are useful indicators of cell health and vitality, especially when used together with a probe for cell membrane integrity like an impermeant nucleic acid stain or fixable live/dead probe.
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3 Calcein Blue AM antibodies from over 3 suppliers available with over 1 conjugates.

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