DAPI / DAPI / N/A
Product Details
| Description | DAPI is a fluorescent dye that binds nucleic acids and demonstrates a high affinity for DNA. It is used extensively in fluorescence microscopy, flow cytometry and microplate assays. DAPI is cell permeable and is readily used for detecting both live, dead and fixed cells. DAPI emits in the blue spectral range and is ideal for multiplexed assays inclusive of green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Aside from labeling cell nuclei, DAPI is also used for the detection of mycoplasma or viral DNA in cell cultures. Wavelength Maxima: Excitation 358nm, Emission 461nm | |
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| Conjugate | DAPI | |
| Clone | N/A | |
| Target Species | All Species | |
| Applications | FC | |
| Supplier | Enzo Life Sciences | |
| Catalog # | Sign in to view product details, citations, and spectra | |
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About DAPI
DAPI (4',6-diamidino-2-phenylindole (DAPI) is a blue-emitting fluorogenic DNA-binding dye that can be excited by the 355 nm blue laser and captured with a 450/50 nm bandpass filter. DAPI has an excitation peak at 350 nm and an emission peak at 465 nm. DAPI is a commonly used DNA stain that binds to AT regions and has many favorable properties. DAPI's fluorescence increases upon binding to DNA, which is beneficial for signal to background ratios. Additionally, DAPI passes through both dead and live cell membranes, with varied success. It passes through dead cell membranes much more effectively making it better for fixed, dead cell staining. It can be used with live cells, but this requires a higher concentration of the dye or can be used to detect membrane depolarization in apoptosis. DAPI is a generic dye sold by many suppliers.
DAPI (4',6-diamidino-2-phenylindole (DAPI) is a blue-emitting fluorogenic DNA-binding dye that can be excited by the 355 nm blue laser and captured with a 450/50 nm bandpass filter. DAPI has an excitation peak at 350 nm and an emission peak at 465 nm. DAPI is a commonly used DNA stain that binds to AT regions and has many favorable properties. DAPI's fluorescence increases upon binding to DNA, which is beneficial for signal to background ratios. Additionally, DAPI passes through both dead and live cell membranes, with varied success. It passes through dead cell membranes much more effectively making it better for fixed, dead cell staining. It can be used with live cells, but this requires a higher concentration of the dye or can be used to detect membrane depolarization in apoptosis. DAPI is a generic dye sold by many suppliers.
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7 DAPI antibodies from over 7 suppliers available with over 1 conjugates.
