IGFBP-5 / Biotin / Polyclonal

Product Details

Description	To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. To detect hIGF-BP5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech's Polyclonal Anti-Human IGF-BP5 (500-P232) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIGF-BP5. To detect hIGF-BP5 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIGF-BP5.Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP5. Anti-Human IGF-BP5 specific antibody was purified by affinity chromatography and then biotinylated.
Conjugate	Biotin
Clone	Polyclonal
Target Species	Human
Applications	ELISA, Capture, WB
Supplier	PeproTech
	Catalog #	Sign in to view product details, citations, and spectra
	Size
	Price
	Antigen
	Host
	Isotype

About IGFBP-5
Enables insulin-like growth factor I binding activity. Involved in several processes, including cellular response to cAMP; regulation of smooth muscle cell migration; and regulation of smooth muscle cell proliferation. Part of insulin-like growth factor ternary complex. Biomarker of pulmonary fibrosis. [provided by Alliance of Genome Resources, Apr 2022]

Experiment Design Tools