Midkine / Biotin / Polyclonal

Product Details

Description	To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMidkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. To detect hMidkine by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech's Polyclonal Anti-Human Midkine (500-P171) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMidkine. To detect hMidkine by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMidkine.Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMidkine. Anti-Human Midkine specific antibody was purified by affinity chromatography and then biotinylated.
Conjugate	Biotin
Clone	Polyclonal
Target Species	Human
Applications	ELISA, Capture, WB
Supplier	PeproTech
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About Midkine
This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]

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