CXCR4 / APC / 12G5

Product Details

Description	CXCR4 is a G-protein coupled receptor that binds the chemokine CXCL121. Chemokines are small 8-12 kDa proteins that mediate cell migration and arrest, homing and trafficking of leukocytes in bone marrow and lymphoid organs, tissue formation, cytoskeletal rearrangement, and immune cell recruitment to inflammation. Additionally, chemokines are expressed by cancer cells, where they enhance tumor angiogenesis and development. CXCR4 is the chemokine receptor most abundantly expressed2 and most frequently detected3 in various cancer types, being present in malignant cell subpopulations in primary tumors as well as sites of metastasis. CXCR4 is involved in tumor cell proliferation and migration2 and is involved in leukocyte chemotaxis in several autoimmune diseases1. CXCR4 also acts as an alternative receptor for some isolates of HIV-2 in the absence of CD44. CXCR4 expression is regulated by HIF-1alpha, IL-5, IFN-gamma, TGF-beta, and IL-17A1. 12G5 was produced by immunizing Balb/c mice with CP-MAC-infected Sup-T1 cells4. Hybridomas were generated and screened for the ability to inhibit CP-MAC-induced syncytium induction on Sup-T1 cells. 12G5 binds specifically to both human and nonhuman cells that express recombinant CXCR44. 12G5 inhibits CD4-independent infection by some HIV-2 isolates, and preincubating cells with 12G5 abolishes syncytium formation. HIV-2/vcp-infected cells display a marked and selective reduction in 12G5 binding. 12G5 also inhibits induction of cell-to-cell fusion of CXCR4+ RD/CD4 cells by HIV-1 and HIV-2 strains5.
Conjugate	APC
Clone	12G5
Target Species	Human
Applications	FC
Supplier	Leinco
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About CXCR4
This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

About APC
Allophycocyanin (APC) is a fluorescent protein derived from cyanobacteria and red algae and a potent donor fluorophore to create tandem dyes that can be excited off the 633-640 nm laser. APC has an excitation peak at 650 nm and a emission peak at 660 nm.

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