About Spark Red 718
Spark Red™ 718 is a brighter alternative to Alexa Fluor® 700, adding to the fluorophore options for the red laser that can detect antigens with lower expression levels. It can be used on a conventional cytometer with a 633 nm red laser and 720/45 filter set (or equivalent filter used to detect Alexa Fluor® 700) spectral cytometers with appropriate instrument settings. On conventional cytometers, Spark Red™ 718 can be utilized with both APC and APC/Cyanine7. On spectral cytometers, it can be unmixed from APC, Spark NIR™ 685, Alexa Fluor® 660, APC/Cyanine7, and APC/Fire™ 810. As spectral properties of Spark Red™ 718 and Alexa Fluor® 700 are nearly identical, using them together in a multicolor panel is not recommended. With a medium brightness, Spark Red™ 718 is best suited for detecting antigens with low to moderate expression levels.