Synaptophysin / FITC / REAL734

Product Details

Description	Clone REAL734 is an antibody fragment derived from the full Synaptophysin antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity. \| Clone REAL734 recognizes protein Synaptophysin, also known as the SYP and major synaptic vesicle protein p38, that occurs in presynaptic vesicles of neurons and in similar vesicles of the adrenal medulla. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations. It is present in neuroendocrine cells and almost all neurons in the brain and spinal cord that participate in synaptic transmission. \| Synaptophysin is also involved in the regulation of short-term and long-term synaptic plasticity and acts as a marker for nerve terminals and for differentiating neuroendocrine tumors. It is reported, that Synaptophysin may provide a marker of breast cancer. Mutations in the gene can result in mental retardation, X-linked 96. \| For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).
Conjugate	FITC
Clone	REAL734
Target Species	Human
Applications	MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier	Miltenyi Biotec
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About Synaptophysin
This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with an X-linked form of cognitive disability. [provided by RefSeq, Jul 2017]

About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

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